Peptide Protocol IQ

Glossary

Research-methodology and pharmacology terms, defined in plain language. Understanding these is most of what separates reading research from being persuaded by it.

B

Beyond-use date
The date after which a prepared solution should not be used, distinct from a manufacturer's expiry date for an unopened product.
Reconstitution
Bioavailability
The proportion of an administered quantity that reaches systemic circulation intact. Peptides administered orally typically have very low bioavailability because they are degraded in the gastrointestinal tract, which is why route matters so much in this category.
PharmacokineticsRoute of administration
Biomarker
A measurable biological characteristic. Moving a biomarker is easier than changing an outcome, and the two should never be reported as if they were equivalent.
Surrogate endpoint
Blinding
Concealment of group allocation from participants, investigators, or both. Without it, expectation can influence both what participants report and how investigators measure it.
Randomised controlled trial

C

Certificate of analysis
A document reporting analytical testing of a specific batch. Its value depends on whether the testing laboratory is independent and whether the certificate corresponds to the batch actually received.
Concentration
Mass per unit volume, for example micrograms per millilitre. Two containers holding the same total mass have different concentrations if different solvent volumes were used.
Reconstitution
Confidence
A separate axis from evidence level, describing how settled the finding is. A serious hypothetical concern and a well-established mild one are different things, and collapsing them into one score loses that distinction.
Evidence level
Contraindication
A situation in which a substance should not be used, as stated in authoritative labelling. We show contraindications only when an authoritative source states one, and we never infer them.
Interaction

D

DOI
Digital Object Identifier, a permanent identifier for a published work that resolves even if the publisher's URL changes.
PMID

E

Endpoint
The outcome a study was designed to measure. A study can only support a conclusion about its own endpoints, and a primary endpoint carries far more weight than a secondary or exploratory one.
Surrogate endpoint
Evidence level
Our A through X scale describing how strong the underlying research is for one specific claim. It is graded per claim, never per compound, because the same compound can have strong evidence for one endpoint and none for another.
Confidence

G

GH axis
The hypothalamic-pituitary pathway governing growth hormone. GHRH analogues act on the pituitary receptor; ghrelin-receptor secretagogues act on a different receptor in the same axis.
IGF-1
GIP
Glucose-dependent insulinotropic polypeptide. Its contribution to the effect of dual agonists remains a subject of scientific debate.
Incretin
GLP-1
Glucagon-like peptide-1. Receptor agonists in this class have the largest randomised evidence base of any compound category in this catalogue.
Incretin

H

Half-life
The time for the concentration of a substance to fall by half. After roughly five half-lives, about 97% has been eliminated. Half-life determines how often a substance was administered in a study.
PharmacokineticsSteady state

I

IGF-1
Insulin-like growth factor 1, produced largely in the liver in response to growth hormone. Frequently measured as a proxy for growth hormone exposure over time.
GH axisBiomarker
In vitro
In cell culture or a test system rather than a living organism. An in-vitro result establishes that something can happen in that system, not that it happens in a person.
Preclinical
Incretin
Gut hormones, principally GLP-1 and GIP, released after eating that augment insulin secretion in a glucose-dependent way and influence gastric emptying and appetite.
GLP-1GIP
Interaction
A situation in which one substance affects another's behaviour or effect. An identified interaction does not establish harm, and the absence of an identified interaction does not establish safety.
Contraindication

L

Lyophilised
Freeze-dried. Many peptides are supplied as a lyophilised powder because the dry form is more stable than the solution.
Reconstitution

M

Meta-analysis
Statistical pooling of results from multiple studies. Quality depends entirely on the studies included; pooling weak or heterogeneous studies does not produce a strong conclusion.
Systematic review

N

NCT number
The registration identifier for a study on ClinicalTrials.gov. Registration before enrolment is what makes it possible to detect selectively reported results.
PMID

P

Pharmacokinetics
What the body does to a substance: absorption, distribution, metabolism, and elimination. Distinct from pharmacodynamics, which is what the substance does to the body.
Half-lifeBioavailability
Placebo
An inactive comparator. Placebo groups often show measurable change, which is why a result without a placebo comparison says very little.
Randomised controlled trial
PMID
PubMed Identifier, a unique number for a record indexed in PubMed. Where we have verified one it links directly to the source; where we have not, we say so rather than publishing an identifier that may not resolve.
DOINCT number
Preclinical
Research conducted in animals or in cell culture, before human study. The large majority of preclinical findings do not translate to humans, which is why we separate this evidence rather than blending it.
In vitroTranslation

R

Randomised controlled trial
A study in which participants are allocated to groups by chance. Randomisation is what allows a difference between groups to be attributed to the intervention rather than to how participants were selected.
BlindingPlacebo
Reconstitution
Dissolving a lyophilised (freeze-dried) solid in a solvent to produce a solution of known concentration. Concentration equals the mass of material divided by the volume of solvent.
ConcentrationLyophilised
Research use only
A labelling phrase. It is not a legal exemption. FDA has taken enforcement action where such labelling was contradicted by website evidence of intended human use, so the phrase does not change what a product actually is.
Route of administration
How a substance enters the body. Topical, oral, subcutaneous, intramuscular, intranasal, and intravenous routes can produce completely different exposure from the same quantity. Evidence from one route does not transfer to another.
Bioavailability

S

Steady state
The point at which the amount entering the body over an interval equals the amount eliminated. Reached after roughly five half-lives of repeated administration at a constant interval.
Half-life
Surrogate endpoint
A measurable substitute for an outcome that matters. A biomarker change is a surrogate; it is not the same as the outcome it stands in for, and surrogates have repeatedly failed to predict real outcomes.
EndpointBiomarker
Systematic review
A structured review using a prespecified search and inclusion strategy. The prespecification is what distinguishes it from a narrative review that selects supportive studies.
Meta-analysis

T

Translation
The step from animal or laboratory finding to human effect. Most compounds that work in animal models do not work in humans, and animal quantities expressed per kilogram do not convert to human quantities by simple arithmetic.
Preclinical